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DCL6B100

Sigma-Aldrich

DEAE–Sepharose™

CL-6B

Synonym(s):

Diethylaminoethyl–Sepharose™

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About This Item

CAS Number:
57407-08-6
MDL number:
MFCD00146630
UNSPSC Code:
47101511
NACRES:
NA.56

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm

pore size

~4,000,000 Da exclusion limit

pH

3—12

capacity

130-170 μeq/mL binding capacity (gel volume)(gel volume)

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General description

DCL6B100-500ML′s updated product number is GE17-0710-01

Application

DEAE-Sepharose® is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Legal Information

DEAE-Sepharose is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R K Sinha et al.
Vaccine, 15(6-7), 689-699 (1997-04-01)
Six different secretory proteins of molecular weights (15, 26, 30, 41, 55 and 70 kDa) were isolated from 8-day-old culture filtrate of Mycobacterium tuberculosis H37Ra using different column chromatography techniques. These proteins were further examined for their ability to induce
I Yu Bakunina et al.
Biochemistry. Biokhimiia, 67(6), 689-695 (2002-07-20)
An alpha-N-acetylgalactosaminidase IV able to remove blood type specificity of human A(II)-erythrocytes and not effecting B(III)-erythrocytes was isolated from the marine bacterium Arenibacter latericius KMM 426T. The alpha-N-acetylgalactosaminidase IV preparation exhibits high activity during inhibition of hemagglutination with blood group
I A Oussenko et al.
Journal of bacteriology, 182(9), 2639-2642 (2000-04-13)
Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major 3'-to-5' exoribonuclease activity in cell extracts, is viable. Here, we show that the
Preparative affinity precipitation of L-lactate dehydrogenase.
Pearson, J.C., et al.
Journal of Biotechnology, 11(2-3), 267-274 (1989)
M Vasseur
Bioscience reports, 9(3), 341-346 (1989-06-01)
The rabbit intestinal sucrase-isomaltase complex has been purified to homogeneity after solubilization with Triton X 100 followed by chromatography on DEAE Sepharose CL 6B and a second solubilization with papain. After hydrophobic chromatography on Octyl Sepharose CL 6B, separation from
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