To reconstitute item D1411, d-Desthiobiotin, use DMSO at a concentration of 62.5 mg/mL (291.70 mM). For improved solubility, warm the tube at 37 ℃ and shake it in an ultrasonic bath. The stock solution can be stored at temperatures below -20℃ for several months.
D1411
d-Desthiobiotin
≥98% (TLC)
Synonym(s):
5-Methyl-2-oxo-4-imidazolidinehexanoic acid
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About This Item
Empirical Formula (Hill Notation):
C10H18N2O3
CAS Number:
Molecular Weight:
214.26
PubChem Substance ID:
eCl@ss:
34058011
UNSPSC Code:
12352200
NACRES:
NA.25
EC Number:
208-566-2
MDL number:
Assay:
≥98% (TLC)
Form:
powder
Pricing and availability is not currently available.
Quality Segment
assay
≥98% (TLC)
form
powder
storage temp.
2-8°C
SMILES string
C[C@@H]1NC(=O)N[C@@H]1CCCCCC(O)=O
InChI
1S/C10H18N2O3/c1-7-8(12-10(15)11-7)5-3-2-4-6-9(13)14/h7-8H,2-6H2,1H3,(H,13,14)(H2,11,12,15)/t7-,8+/m0/s1
InChI key
AUTOLBMXDDTRRT-JGVFFNPUSA-N
Application
d-Desthiobiotin has been used as a component of elution buffers:
- to elute the precipitated proteins from the resin during coimmunoprecipitation[1]
- to elute human Cdc45–MCM–GINS (CMG) helicase during chromatographic protein purification[2]
- to elute the heterodimeric kinesin family member 3A/B (KIF3A/B) and kinesin associated protein 3 (KAP3)-binding APC armadillo (APCARM) domain during chromatographic purification[3]
d-Desthiobiotin is used in affinity chromatography and protein chromatography. d-Desthiobiotin can be used for protein labeling, detection and isolation.
Biochem/physiol Actions
Desthiobiotin is a non-sulfur containing biotin derivative that binds less strongly to biotin-binding proteins and is easily dislocated by biotin.[4]
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This Item | |||
|---|---|---|---|
| assay ≥98% (TLC) | assay ≥98% (HPLC) | assay ≥98% (TLC) | assay - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level - |
| form powder | form powder | form powder | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the recommended method for reconstituting item D1411, d-Desthiobiotin? Also, how should stock solutions be stored?
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