C-12917
Human M2 Macrophages (hMDM-MCSF(-))
Monocyte-derived, single donor, 5 million cryopreserved cells
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human blood (monocyte)
Morphology:
(macrophages)
biological source
human blood (monocyte)
product line
PromoCell®
packaging
pkg of 500,000 cells
morphology
(macrophages)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
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Application
Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 5 million viable cells after thawing.
Biochem/physiol Actions
Monocyte
Preparation Note
Click here for more information.
Other Notes
Recommended Plating Density: 200000 cells per cm2Tested Markers: CD163 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.
Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link
Legal Information
PromoCell is a registered trademark of PromoCell GmbH
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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