B4436

Anti-53BP1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-TP53BP1, Anti-p53 Binding Protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD09039188
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, IP, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 345 kDa
• species reactivity: mouse (predicted), rat (predicted), human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using HEK 293-T cell lysates, microarray: suitable, western blot: 2-4 μg/mL using U2OS total cell extracts
• UniProt accession no.: Q12888
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TP53BP1(7158) ; mouse ... Trp53bp1(27223) ; rat ... Tp53bp1(296099)

Description

General description

Tumor protein p53 binding protein (T153BP1) is mapped to human chromosome 15q15.3. It has methylated histone-binding module and tandem tudor domains.

Immunogen

synthetic peptide corresponding to amino acids 1955-1972 of human 53BP1, conjugated to KLH via an N-terminal added cysteine residue. This sequence is conserved in mouse, and differs from rat by one amino acid

Application

Anti-53BP1 (C-terminal) antibody produced in rabbit has been used in western blotting[1] and immunoprecipitation

Biochem/physiol Actions

Tumor protein p53 binding protein (T153BP1) has a close link to ataxia-telangiectasia mutated (ATM)-regulated events. 53BP1 becomes phosphorylated by ATM in a DNA damage-dependent manner suggesting that it participates in propagating the ATM signaling to its downstream effectors. T153BP1 also plays a key role in DNA repair by mediating nonhomologous end-joining (NHEJ) near double stranded breaks (DSBs). Fusion of T153BP1 with platelet-derived growth factor receptor beta (PDGFRβ) is implicated in a type of myeloproliferative disorder accompanied with eosinophilia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable