The cross-reaction of this antibody with AQP1 has not been tested. The immunogen sequence used to make Product No. A7310, Anti-Water Channel Aquaporin 2, is the rat AQP2 sequence: RQSVELHSPQSLPRGSKA. There is no homology between this sequence and rat AQP1, so cross-reaction would not be expected.
A7310
Anti-Water Channel Aquaporin 2 antibody produced in rabbit
IgG fraction of antiserum, lyophilized powder
Synonym(s):
Anti-Aquaporin 2, Aquaporin 2 Detection, Rabbit Anti-Aquaporin 2
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
30
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable, western blot: 1:200-1:1000 using using Rat Kidney membranes
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AQP2(359)
mouse ... Aqp2(11827)
rat ... Aqp2(25386)
General description
Aquaporin 2 (AQP2) exists as a tetramer with C terminus showing multiple conformations and binds two cadmium ions. AQP2 gene is mapped to human chromosome 12q13.12. It is associated with intracellular vesicles.
Aquaporin 2 (AQP2) is a water channel protein that regulates water balance in renal connecting tubules and collecting ducts. AQP2 is activated by anti-diuretic hormone and subsequently facilitates water inflow in renal cells. This protein also interacts with integrin to modulate epithelial cell morphogenesis and cell movement . Anti-Water Channel Aquaporin 2 antibody recognizes aquaporin 2 from rat by immunoblotting. The antibody also reacts with mouse and human AQP2.
Immunogen
synthetic peptide corresponding to amino acids 254-271 of rat or mouse aquaporin 2 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde. The epitope is identical in rat and mouse and highly conserved in human.
Application
Anti-Water Channel Aquaporin 2 antibody is also suitable for use in chemiluminescent western blot (1:200-1:1000), and immunoblot.
Anti-Water Channel Aquaporin 2 antibody produced in rabbit has been used in:
- immunostaining
- immunohistochemistry
- immunofluorescence
- immunoblotting
Immunohistochemistry was performed on 10% formalin-fixed parraffin embedded rat kidney sections using rabbit anti-AQP2 at a concentration of 5μg/ml. Use of anti-AQP2 allowed visualization of the medullary thick ascending limb and collecting ducts.
Biochem/physiol Actions
Aquaporin (AQP2) N-terminal region plays a key role in trafficking. The C-terminal phosphorylation is crucial for the water permeability function of the apical membrane. Also, ubiquitination at lysine 270 is needed for AQP2 sorting and degradation in lysosomes or elimination through exosomes. Mutation in AQP2 gene is implicated in congenital nephrogenic diabetes insipidus (NDI), a water balance disorder. The functional dysregulation of AQP2 is also correlated to preeclampsia, congestive heart failure and liver cirrhosis.
Physical form
Lyophilized from a solution containing phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide.
Preparation Note
Isolated on immobilized Protein A
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description IgG fraction of antiserum, lyophilized powder | description lyophilized powder, affinity isolated antibody | description affinity isolated antibody | description affinity isolated antibody |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Gene Information human ... AQP2(359) | Gene Information human ... AQP4(361) | Gene Information human ... AQP2(359) | Gene Information mouse ... Aqp3(11828) |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Does the Product No. A7310, Anti-Water Channel Aquaporin 2 antibody, cross-react with the AQP1 protein?
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