93337

Trizma^® acetate

BioUltra, ≥99.0% (NT)

• Synonym(s): TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt
• Linear Formula: NH₂C(CH₂OH)₃ · CH₃COOH
• CAS Number: 6850-28-8
• Molecular Weight: 181.19
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 57653535
• EC Number: 229-939-6
• Beilstein/REAXYS Number: 3702918
• MDL number: MFCD00038951

Properties

• product line: BioUltra
• Quality Segment: 300
• assay: ≥99.0% (NT)
• form: crystalline powder
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.2%
• loss: ≤0.2% loss on drying, 20 °C (HV)
• pH: 6.0-7.0 (25 °C, 0.5 M in H₂O)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.07, λ: 280 nm A_max: 0.05
• SMILES string: CC(O)=O.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)
• InChI key: PIEPQKCYPFFYMG-UHFFFAOYSA-N

Description

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)