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86C

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast blue BB salt

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.47
eCl@ss:
42010105
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Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast blue BB. The resulting insoluble diffuse, blue dye deposit indicates sites of alkaline phosphatase activity.

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This Item
86R85L185L2
application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500, 600

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use


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Kit Components Only

Product No.
Description

  • Citrate Solution (915) 50 mL

  • FBB-Alkaline Solution (863) 10 mL

  • Naphthol AS-BI Alkaline Solution (861) 10 mL

  • Neutral Red Solution (N6264) 50 mL

  • Sodium Nitrite Solution (914) 10 mL

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

wgk

WGK 3

Storage Class

10 - Combustible liquids



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Ming-Tzu Tsai et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 27(9), 1169-1174 (2009-03-11)
Human mesenchymal stem cells (hMSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications by virtue of their capacity for self-renewal and multipotent differentiation. Our intent was to characterize the effect of pulsed electromagnetic fields (PEMFs)
Fatemeh Emamdoust et al.
Animal reproduction, 18(4), e20200257-e20200257 (2022-01-18)
The access to sufficient numbers of spermatogonial stem cells (SSCs) is a prerequisite for the study of their regulation and further biomanipulation. Rho kinase (ROCK) belongs to a family of serine/threonine kinases and involves in a wide range of fundamental
Barbara Klotz et al.
PloS one, 7(1), e29959-e29959 (2012-01-14)
1,25-dihydroxyvitamin D3 (1,25D3) was reported to induce premature organismal aging in fibroblast growth factor-23 (Fgf23) and klotho deficient mice, which is of main interest as 1,25D3 supplementation of its precursor cholecalciferol is used in basic osteoporosis treatment. We wanted to



Global Trade Item Number

SKUGTIN
86C-1KT04061833042328

Questions

  1. Can the 86C or 86R kits be used to detect Alkaline Phosphatase in cell culture media?

    1 answer
    1. The 86C and 86R kits are intended for use with adherent cells. The same applies to SCR004. The APF kit, a fluorescence-based kit, would be more suitable for use if trying to detect alkaline phosphatase in culture media.

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