71013BC

GDS Top 6 STEC (eae) Tq

BioControl, Molecular based PCR test for the detection of the eae gene in "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

Properties

• shelf life: 6‑12 mo.
• manufacturer/tradename: BioControl Systems 71013-100
• application(s): food and beverages
• storage temp.: 2-8°C

Description

General description

GDS Top 6 STEC Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following select O-serogroups of E. coli: O103, O111, O121, O145, O26 and O45. Assurance GDS utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to these specific 6 O-serogroups prior to genetic analysis for the presence of the eae gene.

Features and Benefits

• Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 6 O groups, samples are analyzed for the presence of (eae) with the GDS Top 6 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the GDS Shiga Toxin Genes (Top 6) assay.
• Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

• Top STEC (eae) Amplification Tubes Tq
• Top 6 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)