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70145

Millipore

Malt Extract Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171605
eCl@ss:
42040102
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
malt extract, 30 g/L
mycological peptone, 5 g/L

packaging

pkg of 100 g
pkg of 2.5 kg
pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
clinical testing
environmental
food and beverages
microbiology

suitability

selective by low pH for Aspergillus spp.
selective by low pH for Candida spp.
selective by low pH for Penicillium spp.
selective by low pH for Pichia spp.
selective by low pH for Saccharomyces spp.
selective by low pH for Zygosaccharomyces spp.
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Related Categories

General description

Malt extract agar is considered as a general growth media due to their richness and nutrient balance. It is suitable for the cultivation of fastidious microorganisms. With acidic pH, they are used for the isolation, cultivation and maintenance of yeast and molds. Malt extract contains polysaccharides which are used as energy source. Mycological peptone serves as a nitrogen source and agar is the solidifying agent rapidly yielding a luxuriant growth with typical morphology and pigmentation. Lactic acid, tartaric acid or antibiotics can be added to suppress bacterial growth and make the medium more selective. Reiss recommends a modified malt extract medium for the selective cultivation of Aspergillus flavus. According to Rapp, addition of certain indicator dyes to malt extract agar allows differentiation of yeast and bacterial colonies.

Application

Malt Extract Agar is recommended for the detection, isolation and enumeration of fungi, particularly yeasts and molds, in various materials and for the cultivation of the strains for microbiological vitamin assays

Preparation Note

Suspend 50 g in 1 litre of distilled water and bring to the boil to dissolve. Sterilize by autoclaving at 115°C for 10 minutes. For inhibition of bacterial growth, 2 ml of a 10% sterile filtered lactic acid can be added to adjust the pH to 3.5.

Other Notes

Biological attributes of colony-type variants of Candida albicans; Volatile metabolites and other indicators of P. aurantiogriseum - growth on different substrates

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL9932
BCCK3273
BCCH1946
BCCF6776
BCCC6762

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S Dutton et al.
Journal of general microbiology, 135(12), 3363-3372 (1989-12-01)
Twenty 'commensal' oral or 'pathogenic' vaginal isolates of Candida albicans were examined for colony morphology on malt/yeast-extract and serum-based agar media. Diverse and variable colony morphology was seen on serum agar. In 17 strains, selective subculture of morphologically atypical colonies
T Börjesson et al.
Applied and environmental microbiology, 56(12), 3705-3710 (1990-12-01)
Penicillium aurantiogriseum Dierckx was cultivated on six agar substrates (barley meal agar, oat meal agar, wheat meal agar, malt extract agar, Czapek agar, and Norkrans agar) and on oat grain for 5 days in cultivation vessels provided with an inlet
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier

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