SCC494

Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

Mouse

• Synonym(s): OP9-DL4;OP9-DL4 cell line;DL4 cell line;Delta-like 4 cell line;Notch ligand cell line
• UNSPSC Code: 41106514
• NACRES: NA.45
• Biological source: mouse
• Manufacturer/tradename: Millipore

Properties

• Product Name: Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line,
• biological source: mouse
• Quality Segment: 100
• packaging: vial of ≥1X10⁶ cells
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable, cell differentiation: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

Application

• Each vial contains > 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Biochem/physiol Actions

OP9-DL4 is a stable bone marrow derived stromal cell line expressing the Notch ligand- Delta like 4 (DL4) ectopically.Notch signaling controls multiple cell-fate decisions. Four Notch receptors (Notch 1- Notch 4) have been identified in mammals. These receptors interact with Jagged or Delta-like (DL) family members of Notch ligands. This is followed by cleavage of the intracellular domain of Notch and its subsequent translocation into the nucleus, where it binds with transcription factors and activates transcription of various downstream target genes. Notch signaling is essential in early T- cell lineage commitments. Bone marrow progenitor cells expressing constitutively-active Notch develop into CD4 and CD8 double positive T cells rather than B cells. They also play an important role in development of CD4+ and CD8+ single positive cells from double positive precursor thymocytes, in addition to development of T-cells with αβ+ TCRs. Of the 4 Notch receptors, Notch1 (N1) receptor signaling has been shown to be sufficient for T cell development. The delta like ligands are physiologically relevant N1 ligands. DL1 interacts with both N1 and Notch 2 (N2) to induce T- cell lineage commitment. However, DL4 interacts specifically only with N1 and supports T cell commitment and maturation both in vitro and in vivo. Moreover, results from previous binding studies show that binding between DL1 and N1 is weak as opposed to the stronger binding between DL4 and N1. Of the DL1 and DL4 delta like ligands, DL4 exhibits greater capacity to activate the Notch pathway in hematopoietic progenitor cells. SourceOP9-DL4 cells were genetically modified from OP9 stromal cells derived from mouse bone marrow.References1.Immunity 2002, 17(6): 749-756.2.J Orthop Sci. 2005, 10(6): 589-594.3.Curr Opin Immunol. 2007, 19(2): 163-168. 4.Cold Spring Harb Protoc. 2009, 2009(2): pdb.prot5156.

Features and Benefits

Mouse OP9-DL4 cells express the Notch ligand Delta-like 4 and have been demonstrated to promote T cell lineage commitment in hematopoietic stem cells. These cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. The OP9-DL4 cell line is an essential tool for elucidating Notch signaling in cellular differentiation and the initiation of inflammatory disease states.

Preparation Note

OP9-DL4 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable