MABE645
Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161
clone RM161, from rabbit
Synonym(s):
Histone H3.3, Histone H3
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
RM161, monoclonal
Species reactivity:
human
Application:
WB, multiplexing
Citations:
1
biological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
RM161, monoclonal
species reactivity
human
technique(s)
multiplexing: suitable, western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys9)
Gene Information
human ... H3F3B(3021)
General description
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
A linear peptide corresponding to human Histone H3 acetylated at Lys9.
Application
This Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.
Biochem/physiol Actions
This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in Histone H3.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.
Other Notes
Concentration: Please refer to lot specific datasheet.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.