The anti c-FOS antibody is supplied at a concentration of 1 mg/ml.
ABE457
Anti-c-Fos Antibody
rabbit polyclonal
Synonym(s):
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, rat
Application:
IHC, WB
Citations:
225
Pricing and availability is not currently available.
Product Name
Anti-c-Fos Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
species reactivity (predicted by homology)
sheep (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FOS(2353)
General description
c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.
Application
Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Biochem/physiol Actions
This antibody recognizes the N-terminus of c-Fos.
Analysis Note
Control
PMA(TPA) treated HeLa cell lysate
PMA(TPA) treated HeLa cell lysate
Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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This Item | |||
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| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| biological source rabbit | biological source sheep | biological source mouse | biological source rabbit |
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified antibody | antibody form IgG fraction of antiserum |
| technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue, western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells |
| species reactivity human, rat | species reactivity rat | species reactivity human | species reactivity human |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What is the stock solution concentration for ABE457 (c-fos antibody) used for IHC-related work?
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