ABC131

Anti-Ambra1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 10

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_7662587
• UniProt accession no.: A2AH22
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AMBRA1(55626)

Description

General description

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) is a developmental protein that is involved in autophagy, differentiation and nuerogenesis. AMBRA1 regulates autophagy and development of the nervous system, normal cell survival and proliferation, and the control of protein turnover during neuronal development.

~140 kDa observed. Uniprot describes 7 isoforms at 143 kDa (isoform 1), 133 kDa (isoform 2), 87 kDa (isoform 3), 84 kDa (isoform 4), 100 kDa (isoform 5), 136 kDa (isoform 6), and 130kDa (Isoform 7). Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of mouse Ambra1.

Application

Anti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.5 µg/mL of a representative lot detected Ambra1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of a representative lot detected Ambra1 in human cell lysates (HeLa & U87MG) and in mouse tissue homogenates (liver, kidney, and embryo), as well as exogenously expressed myc-Ambra1 in lysates from transfected HEK293 cells. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: Target specificity of a representative lot was confirmed by Western blotting analysis of Ambra1-knock mouse embryos and HeLa cells treated with Ambra1 siRNA. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: A representative lot detected decreased Ambra1 expression in tissue homogenates (kidney, liver, and lung) from Ambra1+/- mice when compared with tissue samples from wild-type mice. (Cianfanelli, V., et al. (2015). Nat. Cell Biol. 17(1):20-30).

Biochem/physiol Actions

This antibody recognizes the C-terminus of Ambra1. This antibody should recognize isoforms 1 through 5 based upon immunogen design.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF7 cell lysate

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ambra1 in 10 µg of MCF7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable