The antibody is provided as a purified rabbit polyclonal in a buffer. It is recommended to store it at -20ºC in undiluted aliquots for up to 6 months after the date of receipt. It is important to avoid repeated freeze/thaw cycles.
AB5622
Anti-MAP2 Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
Anti-MAP2 antibody, microtubule-associated protein 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat, mouse, human
Application:
ELISA, ICC, IHC (p), WB
Citations:
529
Pricing and availability is not currently available.
Product Name
Anti-Microtubule-Associated Protein 2 (MAP2) Antibody, Chemicon®, from rabbit
biological source
rabbit
Quality Segment
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595)
General description
Approx. 280 kDa doublet (MAP2a and MAP2b) and approx. 70 kDa doublet (MAP2c) on SDS-PAGE.
The microtubule-associated protein 2 (MAP2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). In neurons MAP2 occurs as three primary isoforms, the high molecular weight MAP2a, MAP2b, and the low molecular weight MAP2c, that result from alternative splicing of the MAP2 gene (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP2 together with MAP4 and tau proteins belong to the family of thermostable proteins associated with microtubules.
Immunogen
Purified Microtubule-associated protein from rat brain.
Application
Detect Microtubule-Associated Protein 2 (MAP2) using this Anti-Microtubule-Associated Protein 2 (MAP2) Antibody validated for use in ELISA, IC, IH, IH(P) & WB with more than 55 product citations.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Biochem/physiol Actions
Anti-MAP2 antibody’s reactivity with other species has not been determined. Anti-MAP2 antibody is specific for Microtubule-associated protein 2 (MAP2). The antibody recognizes all MAP2 isoforms (MAP2A, MAP2B, MAP2C and MAP2D). It shows greatest immunoreactivity with the HMW-MAP2 (MAP2A and MAP2B).
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide as a preservative.
Preparation Note
Maintain at -20ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeatedfreeze/thaw cycles.
Analysis Note
Control
Human brain tissue or human glioblastoma T98G cells
Human brain tissue or human glioblastoma T98G cells
Immunohistochemistry(paraffin) Analysis:
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| antibody form saturated ammonium sulfate (SAS) precipitated | antibody form affinity isolated antibody | antibody form purified antibody | antibody form purified immunoglobulin |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... MAP2(4133) | Gene Information human ... MAP2(4133) | Gene Information chicken ... Map2(424001) | Gene Information chicken ... Map2(424001) |
| technique(s) ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunocytochemistry: suitable, western blot: suitable | technique(s) flow cytometry: 1:200-1:500, immunohistochemistry: 1:100-1:200, western blot: 1:500-1:1,000 | technique(s) immunocytochemistry: suitable | technique(s) immunohistochemistry: suitable, western blot: suitable |
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Storage Class
12 - Non Combustible Liquids
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Certificates of Analysis (COA)
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