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AB1555

Sigma-Aldrich

Anti-NMDAR2A Antibody

serum, Chemicon®

Synonym(s):

Anti-EPND, Anti-GluN2A, Anti-LKS, Anti-NMDAR2A, Anti-NR2A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

fish, human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

suitability

not suitable for immunohistochemistry

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GRIN2A(2903)

Specificity

NMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes.

Immunogen

C-Terminal Fusion Protein of the NMDAR2A (30 kDa), aa 1253-1391.

Application

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-NMDAR2A Antibody is validated for use in IP, WB for the detection of NMDAR2A.
Western blotting*: 1:1,000-1:5,000 for ECL. If using labled protein-A for detection: 1:200.

* See suggested protocol

Immunoprecipitation using solubilized hippocampal slices. 3μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200μg rat brain.

Not suggested for use in immunohistochemistry.

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit antiserum. Liquid, no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
NMDAR2A is present in high concentrations in the hippocampus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of repeated prenatal glucocorticoid exposure on long-term potentiation in the juvenile guinea-pig hippocampus.
Setiawan, E; Jackson, MF; MacDonald, JF; Matthews, SG
The Journal of Physiology null
Visual experience-independent functional expression of NMDA receptors in the developing rabbit retina.
Chang, YC; Chen, CY; Chiao, CC
Investigative Ophthalmology & Visual Science null
Zhen-Zhen Duan et al.
Scientific reports, 6, 29246-29246 (2016-07-08)
It has been demonstrated that Src could modulate NMDA receptor, and PAR1 could also affect NMDAR signaling. However, whether PAR1 could regulate NMDAR through Src under ICH has not yet been investigated. In this study, we demonstrated the role of
Benjamin Gronier et al.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 28(1), 211-224 (2017-11-28)
We have previously shown that prebiotics (dietary fibres that augment the growth of indigenous beneficial gut bacteria) such as Bimuno™ galacto-oligosaccharides (B-GOS®), increased N-methyl-D-aspartate (NMDA) receptor levels in the rat brain. The current investigation examined the functional correlates of these
Helene M Savignac et al.
Brain, behavior, and immunity, 52, 120-131 (2015-10-18)
The manipulation of the enteric microbiota with specific prebiotics and probiotics, has been shown to reduce the host's inflammatory response, alter brain chemistry, and modulate anxiety behaviour in both rodents and humans. However, the neuro-immune and behavioural effects of prebiotics

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