740

Wright Stain

Harleco^®, suitable for hematology

• Synonym(s): Hematology Stain
• UNSPSC Code: 41116121
• EC Index Number: 200-659-6

Properties

• form: liquid
• Quality Level: 300
• manufacturer/tradename: Harleco^®
• mp: -97.77 °C
• density: 0.792 g/cm³
• λmax: 520-530 nm (Eosin); 645-655 nm (Azure)
• application(s): diagnostic assay manufacturing; hematology ((contains Methanol))
• shipped in: ambient
• storage temp.: 15-25°C

Description

General description

As a gold standard in hematology, the Harleco^® Wright stain is a Romanowsky neutral dye stain based on the combination of methylene blue azure as the basic dye component and eosin as the acidic dye component. The typical purple color of cell nuclei is due to a molecular interaction between eosin Y and an azure B-DNA complex.

This polychromic stain is widely used to identify acidophilic, basophilic, and neutrophilic granules of leukocytes in peripheral blood and bone marrow smears. For decades, the Harleco Wright stain has been used in clinical diagnostics to characterize red blood cell morphology and to differentiate white blood cell types. The staining result can be influenced by several factors such as pH, buffer composition, fixation, and staining time.

The Harleco Wright stain can be used to stain the following sample materials: fresh, native whole blood or bone-marrow smears and clinical cytology specimens (urine sediment, sputum, smears from fine needle aspiration biopsies (FNAB), rinses, and imprints). It may also be used for G-banding to aid in the analysis of poorly spread metaphases.

HARLECO^® Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.

Application

HARLECO^® Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.

Prepared from dyes certified by the Biological Stain Commission (BSC)
For In-Vitro Diagnostic Use.
For Professional Use.
Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.

Legal Information

HARLECO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225,H301 + H311 + H331,H370
• Precautionary Statements: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Target Organs: Eyes,Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2