NBT is typically soluble in water at 10 mg/mL. Stock solutions at 10mg/ml in water are stable for 1-2 weeks in the dark at 2-8°C. The material has not been tested for solubility in RPMI, rates may be similar to that of water. This compound is also soluble in 2-methoxyethanol at 20 mg/m and ethanol at 5 mg/mL.
484235
p-Nitroblue Tetrazolium Chloride
≥98% (TLC), crystalline solid, nitric oxide synthase inhibitor, Calbiochem®
Synonym(s):
p-Nitroblue Tetrazolium Chloride, NBT, Nitro BT
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About This Item
Empirical Formula (Hill Notation):
C40H30Cl2N10O6
CAS Number:
Molecular Weight:
817.64
UNSPSC Code:
12171500
NACRES:
NA.77
MDL number:
Assay:
≥98% (TLC)
Form:
crystalline solid
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Pricing and availability is not currently available.
Product Name
p-Nitroblue Tetrazolium Chloride, NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM).
Quality Segment
description
RTECS - XF8045000
assay
≥98% (TLC)
form
crystalline solid
potency
3-4 μM IC50
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
yellow
solubility
methanol: soluble, water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
[Cl-].[Cl-].[n+]2([n](nc(n2)c8ccccc8)c3c(cc(cc3)c4cc(c(cc4)[n]5[n+](nc(n5)c7ccccc7)c6ccc(cc6)[N+](=O)[O-])OC)OC)c1ccc(cc1)[N+](=O)[O-]
InChI
1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key
FSVCQIDHPKZJSO-UHFFFAOYSA-L
General description
NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
Biochem/physiol Actions
Primary Target
NOS
NOS
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
Other Notes
Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
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This Item | |||
|---|---|---|---|
| form crystalline solid | form solid | form solid | form solid |
| assay ≥98% (TLC) | assay ≥98% (TLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition OK to freeze, protect from light, desiccated (hygroscopic) | storage condition OK to freeze | storage condition OK to freeze, protect from light | storage condition OK to freeze |
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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