1.10259
DCA agar acc. to Weenk et al.
for microbiology
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About This Item
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Quality Level
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
7.4-7.8 (25 °C in H2O)
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
Clostridium spp.
Analysis Note
Appearance
- clearness:
clear
- colour: yellowish-Brown to reddish-Brown
pH-value (25 °C): 7.4 - 7.8
Growth
- Clostridium perfringens ATCC 10543:
good to very good
- Clostridium perfringens ATCC 13124 (WDCM 00007): good to very good
- Clostridium sporogenes ATCC 19404 (WDCM 00008): good to very good
- Clostridium bifermentans ATCC 19299: good to very good
- Bacillus licheniformis ATCC 14580 (WDCM 00068): poor to fair
Blacking
- Clostridium perfringens ATCC 10543:
+
- Clostridium perfringens ATCC 13124 (WDCM 00007): +
- Clostridium sporogenes ATCC 19404 (WDCM 00008): +
- Clostridium bifermentans ATCC 19299: +
- Bacillus licheniformis ATCC 14580 (WDCM 00068): -
Recovery on test medium
- Clostridium perfringens ATCC 10543:
≥ 70 %
- Clostridium perfringens ATCC 13124 (WDCM 00007): ≥ 70 %
- Clostridium sporogenes ATCC 19404 (WDCM 00008): ≥ 70 %
- Clostridium bifermentans ATCC 19299: ≥ 70 %
Incubation: 48 hrs.; 30 °C with Anaerocult A.
- clearness:
clear
- colour: yellowish-Brown to reddish-Brown
pH-value (25 °C): 7.4 - 7.8
Growth
- Clostridium perfringens ATCC 10543:
good to very good
- Clostridium perfringens ATCC 13124 (WDCM 00007): good to very good
- Clostridium sporogenes ATCC 19404 (WDCM 00008): good to very good
- Clostridium bifermentans ATCC 19299: good to very good
- Bacillus licheniformis ATCC 14580 (WDCM 00068): poor to fair
Blacking
- Clostridium perfringens ATCC 10543:
+
- Clostridium perfringens ATCC 13124 (WDCM 00007): +
- Clostridium sporogenes ATCC 19404 (WDCM 00008): +
- Clostridium bifermentans ATCC 19299: +
- Bacillus licheniformis ATCC 14580 (WDCM 00068): -
Recovery on test medium
- Clostridium perfringens ATCC 10543:
≥ 70 %
- Clostridium perfringens ATCC 13124 (WDCM 00007): ≥ 70 %
- Clostridium sporogenes ATCC 19404 (WDCM 00008): ≥ 70 %
- Clostridium bifermentans ATCC 19299: ≥ 70 %
Incubation: 48 hrs.; 30 °C with Anaerocult A.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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