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Trifluoroacetic acid solution

(25% solution in water) for protein sequenation

Synonym(s):

Trifluoroacetic acid solution, TFA solution, TFA

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About This Item

Linear Formula:
CF3COOH
CAS Number:
76-05-1
Molecular Weight:
114.02
Beilstein:
5446934
MDL number:
MFCD00004169
UNSPSC Code:
12352106
NACRES:
NB.21

Quality Level

200

form

liquid

composition

Water, 74.5-75.5% Karl Fischer

concentration

24.5-25.5% (trifluoroacetic acid)

density

1.12 g/cm3 at 20 °C

storage temp.

15-25°C

SMILES string

OC(=O)C(F)(F)F

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

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Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13

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