1.04179

Brilliant cresyl blue zinc chloride double salt

• Linear Formula: (C₁₇H₂₀N₃O)₂ · ZnCl₄
• CAS Number: 51716-96-2
• Molecular Weight: 771.92
• MDL number: MFCD00148901
• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• composition: Dye, ≥60% spectrophotometric assay
• pH: 5.5 (20 °C, 5 g/L in H₂O)
• mp: 195-198 °C
• solubility: 30 g/L
• bulk density: 560 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: SIHASHFMDFXGSX-UHFFFAOYSA-J

Description

General description

Brilliant cresyl blue zinc chloride double salt for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution, that makes target structures (by staining with the above brilliant cresyl blue solution) evaluable for diagnostic purposes. The regenerative capacity of erythrocytes can be detected by counting of reticulocytes. The measurement of substantia granulofilamentosa (ribonucleoproteins) is possible with fresh, non-fixed, young erythrocytes (supravital staining).Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly. When stained with brilliant cresyl blue the reticulocytes display a black-blue network or black-blue dots. As CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (spectrophotometrically): ≥ 60% C₁₇H₂₀N₃O
Identity
- UV/VIS-Spectrum:
passes test
- Zinc: passes test
Absorption maximum λ max (ethanol 50 %): 623 - 628 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; ethanol 50 %): ≥ 1500
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable