05-481

Anti-phospho-MAP Kinase1/2 Antibody

clone 12D4, Upstate^®, from mouse

• Synonym(s): Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, Microtubule-associated protein 2 kinase, extracellular signal-related kinase 1, mitogen-activated protein kinase 3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 12D4, monoclonal
• Species reactivity: avian, rat, mouse, canine, human
• Application: ELISA, ICC, IHC, IP, WB
• Citations: 36

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 12D4, monoclonal
• species reactivity: avian, rat, mouse, canine, human
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002745, NM_002746
• UniProt accession no.: P27361
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr202/pTyr204)
• Gene Information: human ... MAPK1(5594)

Description

General description

42-44 kDa

Mitogen-activated protein kinase 3 (UniProt: P27632; also known as EC:2.7.11.24, MAP kinase 3, MAPK 3. ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase p44-ERK1) is encoded by the MAPK3 (also known as ERK1, PRKM3) gene (Gene ID: 5595) in human. ERK-1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is expressed in different tissues and its highest levels have been observed in the nervous system. The MAPK/ERK cascade mediates several diverse biological processes such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. It also plays a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a few transcription factors. About 160 substrates have been described for ERKs. The protein kinase domain of ERK-1 is localized to amino acids 42-330 and the TXY motif is located in amino acids 202-204. ERK-1 is phosphorylated by MEK1 and MEK2 on threonine 202 and tyrosine 204 in response to external stimuli like insulin or nerve growth factor and both these phosphorylations are essential for its activity. Three isoforms of ERK-1 have been described that are produced by alternative splicing.

Immunogen

KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4

Application

Detect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC.

Immunoprecipitation: Use 1-10 μg per immuno-precipitation.

Research Category
Signaling

Research Sub Category
MAP Kinases

Biochem/physiol Actions

Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.

Physical form

Format: Purified

Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.

Thiophilic adsoption and size exclusion chromatography

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.

Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.