M-089

(±)-Methadone-D₉ solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®

• Empirical Formula (Hill Notation): C₂₁D₉H₁₈NO
• Molecular Weight: 318.50
• EC Number: 200-659-6
• MDL number: MFCD27966808
• UNSPSC Code: 41116107
• PubChem Substance ID: 329817836
• NACRES: NA.24

Properties

• grade: certified reference material
• form: liquid
• feature: Snap-N-Spike^®/Snap-N-Shoot^®
• packaging: ampule of 1 mL
• manufacturer/tradename: Cerilliant^®
• drug control: Narcotic Licence Schedule A (Switzerland); estupefaciente (Spain); Decreto Lei 15/93: Tabela IA (Portugal)
• concentration: 1.0 mg/mL in methanol
• technique(s): gas chromatography (GC): suitable; liquid chromatography (LC): suitable
• application(s): forensics and toxicology
• format: single component solution
• storage temp.: 2-8°C
• SMILES string: CC(N(C([2H])([2H])[2H])C([2H])([2H])[2H])CC(C1=CC=CC=C1)(C(CC([2H])([2H])[2H])=O)C2=CC=CC=C2
• InChI: 1S/C21H27NO/c1-5-20(23)21(16-17(2)22(3)4,18-12-8-6-9-13-18)19-14-10-7-11-15-19/h6-15,17H,5,16H2,1-4H3/i1D3,3D3,4D3
• InChI key: USSIQXCVUWKGNF-MXOMHCEVSA-N

Description

General description

A stable-labeled internal standard applicable for use in isotope dilution methods or quantitation of methadone levels by GC/MS or LC/MS in forensic analysis, urine drug testing, pain prescription monitoring or clinical toxicology applications. Methadone is a synthetic opioid used medically as an analgesic and as a maintenance anti-addictive for use in patients with opioid dependency. The drug is sold under several trade names such as Symoron, Dolophine^®, Amidone, Methadose^®, Physeptone, or Heptadon.

Application

• Quantitative blood spot analysis: A sensitive LC-MS/MS assay was developed to quantify methaDoneand its metabolites in dried blood spots, demonstrating the application of Methadone-D₉ as an internal standard. This method offers a novel approach for monitoring methaDonelevels in patients, supporting drug metabolism and pharmacokinetic studies (Davari et al., 2020).


• Molecular imprinting technique: A study employed molecularly imprinted polymer-sol-gel technology for micro-solid phase extraction of methadone, utilizing Methadone-D₉ for calibration in human plasma. This highlights its role in enhancing the accuracy of quantitative analyses in pharmaceutical research, particularly in the context of liquid chromatography-tandem mass spectrometry (El-Beqqali and Abdel-Rehim, 2016).


• Forensic toxicology: A validated gas chromatography-mass spectrometry method was developed for simultaneous determination of methaDoneand its metabolites in umbilical cord plasma. Methadone-D₉ served as an internal standard, crucial for accurate measurement and forensic investigations (Nikolaou et al., 2008).

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany

Dolophine is a registered trademark of Roxane Laboratories, Inc.

Methadose is a registered trademark of Mallinckrodt Inc.

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225,H301 + H311 + H331,H370
• Precautionary Statements: P210 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Target Organs: Eyes
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 49.5 °F - closed cup
• Flash Point(C): 9.7 °C - closed cup