Skip to Content
Merck

DNA sequence and analysis of human chromosome 9.

Nature (2004-05-28)
S J Humphray, K Oliver, A R Hunt, R W Plumb, J E Loveland, K L Howe, T D Andrews, S Searle, S E Hunt, C E Scott, M C Jones, R Ainscough, J P Almeida, K D Ambrose, R I S Ashwell, A K Babbage, S Babbage, C L Bagguley, J Bailey, R Banerjee, D J Barker, K F Barlow, K Bates, H Beasley, O Beasley, C P Bird, S Bray-Allen, A J Brown, J Y Brown, D Burford, W Burrill, J Burton, C Carder, N P Carter, J C Chapman, Y Chen, G Clarke, S Y Clark, C M Clee, S Clegg, R E Collier, N Corby, M Crosier, A T Cummings, J Davies, P Dhami, M Dunn, I Dutta, L W Dyer, M E Earthrowl, L Faulkner, C J Fleming, A Frankish, J A Frankland, L French, D G Fricker, P Garner, J Garnett, J Ghori, J G R Gilbert, C Glison, D V Grafham, S Gribble, C Griffiths, S Griffiths-Jones, R Grocock, J Guy, R E Hall, S Hammond, J L Harley, E S I Harrison, E A Hart, P D Heath, C D Henderson, B L Hopkins, P J Howard, P J Howden, E Huckle, C Johnson, D Johnson, A A Joy, M Kay, S Keenan, J K Kershaw, A M Kimberley, A King, A Knights, G K Laird, C Langford, S Lawlor, D A Leongamornlert, M Leversha, C Lloyd, D M Lloyd, J Lovell, S Martin, M Mashreghi-Mohammadi, L Matthews, S McLaren, K E McLay, A McMurray, S Milne, T Nickerson, J Nisbett, G Nordsiek, A V Pearce, A I Peck, K M Porter, R Pandian, S Pelan, B Phillimore, S Povey, Y Ramsey, V Rand, M Scharfe, H K Sehra, R Shownkeen, S K Sims, C D Skuce, M Smith, C A Steward, D Swarbreck, N Sycamore, J Tester, A Thorpe, A Tracey, A Tromans, D W Thomas, M Wall, J M Wallis, A P West, S L Whitehead, D L Willey, S A Williams, L Wilming, P W Wray, L Young, J L Ashurst, A Coulson, H Blöcker, R Durbin, J E Sulston, T Hubbard, M J Jackson, D R Bentley, S Beck, J Rogers, I Dunham
ABSTRACT

Chromosome 9 is highly structurally polymorphic. It contains the largest autosomal block of heterochromatin, which is heteromorphic in 6-8% of humans, whereas pericentric inversions occur in more than 1% of the population. The finished euchromatic sequence of chromosome 9 comprises 109,044,351 base pairs and represents >99.6% of the region. Analysis of the sequence reveals many intra- and interchromosomal duplications, including segmental duplications adjacent to both the centromere and the large heterochromatic block. We have annotated 1,149 genes, including genes implicated in male-to-female sex reversal, cancer and neurodegenerative disease, and 426 pseudogenes. The chromosome contains the largest interferon gene cluster in the human genome. There is also a region of exceptionally high gene and G + C content including genes paralogous to those in the major histocompatibility complex. We have also detected recently duplicated genes that exhibit different rates of sequence divergence, presumably reflecting natural selection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lipase from Mucor miehei, powder, slightly brown, ~1 U/mg
Sigma-Aldrich
Lipase from Candida rugosa, lyophilized, powder (fine), 15-25 U/mg
Sigma-Aldrich
Lipase from Rhizopus oryzae, powder (fine), ~10 U/mg
Sigma-Aldrich
Lipase from Candida rugosa, powder, yellow-brown, ≥2 U/mg
Sigma-Aldrich
Lipase from Aspergillus oryzae, lyophilized, powder, white, ~50 U/mg
Sigma-Aldrich
Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast, Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)
Sigma-Aldrich
Lipase from Mucor miehei, lyophilized powder, ≥4,000 units/mg solid (using olive oil)
Sigma-Aldrich
Myokinase from rabbit muscle, ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)
Sigma-Aldrich
Lipase from porcine pancreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Sigma-Aldrich
Lipase from porcine pancreas, Type VI-S, ≥20,000 units/mg protein, lyophilized powder
Sigma-Aldrich
Lipase from wheat germ, Type I, lyophilized powder, 5-15 units/mg solid
Sigma-Aldrich
Taq DNA Polymerase from Thermus aquaticus, with 10× PCR reaction buffer containing MgCl2
Sigma-Aldrich
Aconitase from porcine heart
Sigma-Aldrich
Cyclooxygenase 1 from sheep, glycerol solution, ≥1500 units/mg protein
Sigma-Aldrich
Lipase acrylic resin, ≥5,000 U/g, recombinant, expressed in Aspergillus niger
Sigma-Aldrich
Lipase from Aspergillus oryzae, ≥20,000 U/g
Sigma-Aldrich
Lipase from Pseudomonas sp., Type XIII, lyophilized powder, ≥15 units/mg solid
Sigma-Aldrich
Taq DNA Polymerase from Thermus aquaticus, with 10× PCR reaction buffer without MgCl2
Sigma-Aldrich
Lipase from Candida rugosa, Type VII, ≥700 unit/mg solid
Sigma-Aldrich
Lipase from Candida rugosa, lyophilized powder, ≥40,000 units/mg protein
Sigma-Aldrich
Lipase from Rhizopus niveus, powder (fine), ≥1.5 U/mg
Sigma-Aldrich
Nucleoside Phosphorylase bacterial, recombinant, expressed in E. coli, ≥10 units/mg protein
Sigma-Aldrich
Lipase from Candida sp., recombinant, expressed in Aspergillus niger
Sigma-Aldrich
Lipase B Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~9 U/mg
Sigma-Aldrich
Lipase A Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~2 U/mg
Sigma-Aldrich
Lipase from Pseudomonas cepacia, powder, light beige, ≥30 U/mg
Sigma-Aldrich
Carnitine Acetyltransferase from pigeon breast muscle, ammonium sulfate suspension, ≥50 units/mg protein
Sigma-Aldrich
DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution
Sigma-Aldrich
Aldolase from rabbit muscle, lyophilized powder, ≥8.0 units/mg protein
Sigma-Aldrich
Galactosyltransferase from bovine milk, lyophilized powder