L3001

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

• Synonym(s): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS Number: 9001-62-1
• Enzyme Commission number: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: wheat (germ)
• type: Type I
• form: lyophilized powder
• specific activity: 5-15 units/mg solid
• UniProt accession no.: Q8L6B0
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N
• Gene Information: wheat ... lipase(543006)

Description

General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:

• to prepare enzyme solution for the biodegradability test
• to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
• to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Quality

Contains acid phosphatase.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO₂ in 30 minutes.)

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves