Y1876
Yeast Synthetic Drop-out Medium Supplements
without tryptophan
Synonym(s):
Yeast Growth Medium
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Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Components
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for tryptophan. All the amino acids are present at a concentration of 76 mg/L except for leucine, which is present at a concentration of 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Amino acids: All standard amino acids except for tryptophan. All the amino acids are present at a concentration of 76 mg/L except for leucine, which is present at a concentration of 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Quantity
1.92 g will supplement 1 liter of medium.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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