T9378
Trypsin inhibitor
crude powder
Synonym(s):
Trypsin Inhibitor from lima beans
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About This Item
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Product Name
Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
biological source
Phaseolus limensis (lima bean)
Quality Level
type
Type II-L
form
crude powder
mol wt
9 kDa
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
storage temp.
2-8°C
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Application
Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
- in the inhibition of trypsin in human plasma
- in the termination of proteolytic digestion in rabbit skeletal muscles
- in the inhibition of salivary glands enzyme extract from Lygus lineolaris
- in the inhibition of proteolytic activity in Agave tequilana enzyme extract
Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.
Biochem/physiol Actions
Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.
Physical properties
Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.
Unit Definition
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
Preparation Note
Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).
Analysis Note
One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
Other Notes
View more information on Trypsin Inhibitor.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Chromatograms
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