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S7776

Sigma-Aldrich

Starch Azure

Insoluble corn starch covalently linked with Remazol Brilliant Blue R

Synonym(s):

Starch–Remazol brilliant blue R, RBB-Starch, Remazol brilliant blue R dyed starch, Starch Azure

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.28

form

powder

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General description

Starch is an insoluble starch substrate specific for α-amylase and not for other exo enzymes. α-amylase catalysis of starch azure solubilization is measured at 595 nm.

Application

Starch Azure has been used as a substrate
  • in the screening of α-amylase activity in decontaminated malted flour at varying pH and temperature
  • for monitoring the inhibitory activity of Physalis peruviana extracts
  • for thermostable α-amylase extracted from deep sea and acidic soil

Substrates

Substrate for colorimetric determination of α-amylase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibitory effects of an extract of fruits of Physalis peruviana on some intestinal carbohydrases
Rey D, et al.
Revista Colombiana de Ciencias Quimico-Farmaceuticas, 44(1), 72-89 (2015)
Modeling cereal starch hydrolysis during simultaneous saccharification and lactic acid fermentation; case of a sorghum-based fermented beverage, gowe
Mestres C, et al.
Food Research International, 100, 102-111 (2017)
The use of starch azure for measurement of alpha-amylase activity
Lehoczki G, et al.
Carbohydrate Polymers, 183, 263-266 (2018)
Gábor Lehoczki et al.
Carbohydrate polymers, 183, 263-266 (2018-01-22)
Despite being widely used, there is no standard protocol for α-amylase activity measurement with starch azure substrate. Boiling pre-treatment of starch azure suspension increased the reaction rate of hydrolysis catalysed by human salivary α-amylase (HSA) or porcine pancreatic α-amylase (PPA)
A novel, high performance enzyme for starch liquefaction discovery and optimization of a low pH, thermostable alpha-amylase
Richardson TH, et al.
The Journal of Biological Chemistry, 277(29), 26501-26507 (2002)

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