RAB0430

Human ShhN ELISA Kit

for serum, plasma, cell culture supernatants and urine

• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type serum; sample type urine; sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 42 pg/mL; standard curve range: 40.96-10000 pg/mL; (Assay Diluent C); inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 8 pg/mL; standard curve range: 40.96-10000 pg/mL; (Assay Diluent B)
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SHH(6469)

Description

General description

The Human Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human Shh-N in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human ShhN

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor patched (Ptc-1), de-represses smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABELADC ELISA 1X Assay/Sample Diluent Buffer C (Item L)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials