P1473
PCR 100 bp Low Ladder
for electrophoresis of PCR fragments
Synonym(s):
100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker
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About This Item
UNSPSC Code:
41141827
NACRES:
NA.25
Recommended Products
grade
for molecular biology
Quality Level
form
liquid
usage
75 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.
Application
PCR 100 bp Low Ladder has been used:
- in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
- to visualize DNA from enzyme digested PCR mixture
- to estimate the molecular weight of the PCR product fragments
Components
Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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