K1757

Keratinocyte Growth Factor human

KGF, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): hFGF-7, hKGF, FGF-7, KGF
• CAS Number: 148348-15-6
• MDL number: MFCD00212245
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE and HPLC)
• form: powder
• potency: 0.1-15 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 18.9 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: 1 EU/μg
• UniProt accession no.: P21781
• storage temp.: −20°C
• Gene Information: human ... FGF7(2252)

Description

General description

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.

Application

Keratinocyte Growth Factor human has been used:

• as a component of epithelial growth medium
• as a positive control for antigen to determine the activity of the antibody
• to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Biochem/physiol Actions

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Determined by a cell proliferation assay using 4MBr-5 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves