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H1387

Sigma-Aldrich

Hanks′ Balanced Salts

Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

HBSS

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salts has been used:
  • for diluting digested tissues during isolation of pancreatic blood vessels
  • for washing mouse intestinal mucosal cells before lysis and sonication
  • as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone

Quantity

Formulated to contain 9.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dajana Milovac et al.
Materials science & engineering. C, Materials for biological applications, 34, 437-445 (2013-11-26)
In the present study, poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone was prepared. Hydrothermal transformation of aragonitic cuttlefish bone into hydroxyapatite (HAp) was performed at 200°C retaining the cuttlebone architecture. The HAp scaffold was coated with a poly(ε-caprolactone) (PCL) using
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