GE28-9042-65
illustra™ Blood Mini , 250
Cytiva 28-9042-65
Synonym(s):
Blood Mini Kit, illustra Blood Kit
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.55
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packaging
pkg of 250 ea
manufacturer/tradename
Cytiva 28-9042-65
capacity
60 μg binding capacity
Related Categories
General description
Blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
Genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μL sample. Input sample volumes can vary from 50 μL to 1 mL.
The method uses a simple genomic DNA purification protocol. This protcol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. This results in high molecular weight DNA that can be used downstream (e.g., in bone marrow genomic DNA for next generation sequencing).
The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.
The genomic DNA kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μL sample. Input sample volumes can vary from 50 μL to 1 mL.
The method uses a simple genomic DNA purification protocol. This protcol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. This results in high molecular weight DNA that can be used downstream (e.g., in bone marrow genomic DNA for next generation sequencing).
The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.
The genomic DNA kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Application
Suitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; restriction enzyme digestion; and genotyping.
Features and Benefits
- High-quality genomic DNA extraction from blood with a greater proportion of higher molecular weight DNA and less shearing
- Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
- Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
- Verified in several downstream applications including reverse transcription polymerase chain reaction (RT-PCR), endpoint PCR, multiplex PCR, and restriction enzyme digests
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
illustra is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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