Skip to Content
Merck
All Photos(3)

Documents

G3548

Sigma-Aldrich

Anti-GADD45A (12-25) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-DDIT1, Anti-GADD45, Anti-Growth arrest and DNA-damage-inducible protein GADD45 α

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~18 kDa

species reactivity

human

technique(s)

western blot: 1:500-1:2,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GADD45A(1647)

General description

Growth arrest and DNA damage inducible α It is expressed at low levels in testis, but, at moderate level in liver, brain, heart and spleen. GADD45A shows high expression in kidney and skeletal muscle.

Immunogen

synthetic peptide corresponding to amino acids 12-25 of human GADD45A

Application

Anti-GADD45A (12-25) antibody produced in rabbit has been used in immunocytochemistry.
Anti-GADD45A antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

GADD45A (growth arrest and DNA-damage-inducible, alpha) gene encodes a protein that belongs to a group of genes that are expressed under stressful growth arrest conditions and upon treatment with DNA-damaging agents such as methylmethane sulfonate and UV radiation. The protein mediates activation of the p38/JNK pathway via MTK1/MEKK4 kinase upon environmental stress. It inhibits entry of cells into S phase by activating DNA excision repair in vitro. It plays a crucial role in cell cycle checkpoints, DNA repair, and genome integrity maintenance.
Growth arrest and DNA damage inducible α (GADD45A) miRNAs are differentially expressed in osteosarcoma. It interacts with proliferating cell nuclear antigen (PCNA), aurora A kinase and histones. GADD45A improves the cytotoxic effect of the drug temozolomide (TMZ) in glioblastoma multiforme (GBM).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ossie Geifman-Holtzman et al.
Advances in experimental medicine and biology, 793, 121-129 (2013-10-10)
Preeclampsia is a pregnancy-induced complex of multiple pathological changes. Numerous stresses during pregnancy, including hypoxia, immune activation, inflammatory cytokines, and oxidative stress were reported as contributing factors to the preeclamptic pathology. Seeking common sensors of various stressors in preeclampsia is
GADD45A plays a protective role against temozolomide treatment in glioblastoma cells
Wang HH, et al.
Scientific Reports, 7(1), 8814-8814 (2017)
Analysis of miRNA-gene expression-genomic profiles reveals complex mechanisms of microRNA deregulation in osteosarcoma
Maire G, et al.
Cancer Genetics, 204(3), 138-146 (2011)
Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase
Sanchez R, et al.
The Journal of Biological Chemistry, 285(29), 22196-22201 (2010)
M C Hollander et al.
The Journal of biological chemistry, 268(32), 24385-24393 (1993-11-15)
The gadd45 gene is transcriptionally activated through at least two different mechanisms; one following treatment with base-damaging agents such as methylmethane sulfonate and UV radiation and the other following ionizing radiation. To investigate the sequences involved in induction of gadd45

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service