G1512
β-Glucuronidase from Helix pomatia
Type H-5, lyophilized powder, ≥400,000 units/g solid
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
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type
Type H-5
form
lyophilized powder
specific activity
≥400,000 units/g solid
secondary activity
≤40,000 units/g solid sulfatase
storage temp.
−20°C
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General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
β-Glucuronidase from Helix pomatia has been used:
- in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
- in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
- in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)
β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Linkage
A further purification of G0876 by gel permeation chromatography.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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