F9679
Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit
1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-FAS, Anti-FASN
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 270 kDa
species reactivity
mouse, rat, human
concentration
1 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using lysates of Rat1 cells
western blot: 1-2 μg/mL using lysates of human HEK 293-T cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FASN(2194)
mouse ... Fasn(14104)
rat ... Fasn(50671)
General description
Fatty acid synthase (FAS, FASN) is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).
Immunogen
synthetic peptide corresponding to amino acids 2497-2511 of human fatty acid synthase, conjugated to KLH via an N-terminal added lysine residue. The corresponding sequence is conserved in human, rat, and mouse.
Application
Anti-Fatty Acid Synthase (C-Terminal) antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of Rat1 cells and 1-2μg/mL using lysates of human HEK 293-T cells.
Biochem/physiol Actions
FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Pricing
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C Rufo et al.
The Journal of biological chemistry, 276(24), 21969-21975 (2001-03-30)
Refeeding carbohydrate to fasted rats induces the transcription of genes encoding enzymes of fatty acid biosynthesis, e.g. fatty-acid synthase (FAS). Part of this transcriptional induction is mediated by insulin. An insulin response element has been described for the fatty-acid synthase
Francisco J Asturias et al.
Nature structural & molecular biology, 12(3), 225-232 (2005-02-16)
De novo synthesis of fatty acids in the cytosol of animal cells is carried out by the multifunctional, homodimeric fatty acid synthase (FAS). Cryo-EM analysis of single FAS particles imaged under conditions that limit conformational variability, combined with gold labeling
DaoHai Zhang et al.
Molecular & cellular proteomics : MCP, 4(11), 1686-1696 (2005-07-29)
The receptor tyrosine kinase ErbB2 (HER-2/neu) is overexpressed in up to 30% of breast cancers and is associated with poor prognosis and an increased likelihood of metastasis especially in node-positive tumors. In this proteomic study, to identify the proteins that
Javier A Menendez et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(29), 10715-10720 (2004-07-06)
Fatty acid synthase (FAS) activity is a potential therapeutic target to treat cancer and obesity. Here, we have identified a molecular link between FAS and HER2 (erbB-2) oncogene, a marker for poor prognosis that is overexpressed in 30% of breast
Fatty acid synthase, a proficient multifunctional enzyme.
S J Wakil
Biochemistry, 28(11), 4523-4530 (1989-05-30)
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