This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.
F1804
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Synonym(s):
flag antibody, Anti-ddddk, Anti-dykddddk
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
Specificity
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting[1], immunoprecipitation (IP) [2][3][4], immunohistochemisty, immunofluorescence[5] and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?
Barbara Lipert
PLoS ONE (2017)
ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
Yumin Qiu et al.
Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as
-
Is this Ab suitable for ELISA?
1 answer-
Helpful?
-
-
Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci
1 answer-
Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
https://www.nature.com/articles/s41467-019-12187-5.pdfHelpful?
-
-
モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
1 answer-
An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdfHelpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
