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D9943

Sigma-Aldrich

Anti-Doublecortin (N-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DCX, Anti-Doublecortex, Anti-LISX, Anti-X-linked lissencephaly, Anti-XLIS

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~40 kDa

species reactivity

human, rat, mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction)
western blot: 1-2 μg/mL using mouse brain extract (S1 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DCX(1641)
mouse ... Dcx(13193)
rat ... Dcx(84394)

General description

Doublecortin gene is mapped to human chromosome Xq23. It comprises two homologous doublecortin (DC) domains, one each in the N-terminal and C-terminal region. A linker connects a flexible unstructured region. The C-terminal sequences also have serine/proline-rich region.

Specificity

Anti-Doublecortin(N-terminal) specifically recognizes human, rat, and mouse Doublecortin.

Immunogen

synthetic peptide corresponding to amino acids 1-17 located at the N-terminus of rat doublecortin, conjugated to KLH. This sequence is identical in mouse, and highly conserved in human doublecortin.

Application

Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), and 1-2μg/mL using mouse brain extract (S1 fraction).

Biochem/physiol Actions

Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cui-Hong Zhou et al.
Frontiers in cellular neuroscience, 13, 275-275 (2019-07-12)
Electroacupuncture (EA) pretreatment is a clinically useful therapy for several brain disorders. However, whether and via which exact molecular mechanisms it ameliorates post-traumatic stress disorder (PTSD) remains unclear. In the present study, rats received EA stimulation for seven consecutive days
Thomas V Wuttke et al.
Nature neuroscience, 21(4), 517-529 (2018-03-07)
Repair of complex CNS circuitry requires newly incorporated neurons to become appropriately, functionally integrated. One approach is to direct differentiation of endogenous progenitors in situ, or ex vivo followed by transplantation. Prior studies find that newly incorporated neurons can establish
J G Gleeson et al.
Neuron, 23(2), 257-271 (1999-07-10)
Doublecortin (DCX) is required for normal migration of neurons into the cerebral cortex, since mutations in the human gene cause a disruption of cortical neuronal migration. To date, little is known about the distribution of DCX protein or its function.
Elio Messi et al.
BMC cancer, 8, 30-30 (2008-01-31)
Neuroblastoma is a severe pediatric tumor, histologically characterised by a variety of cellular phenotypes. One of the pharmacological approaches to neuroblastoma is the treatment with retinoic acid. The mechanism of action of retinoic acid is still unclear, and the development
Maryam Moslehi et al.
Scientific reports, 7(1), 5245-5245 (2017-07-14)
Doublecortin X (DCX), known to be essential for neuronal migration and cortical layering in the developing brain, is a 40 kDa microtubule (MT)-associated protein. DCX directly interacts with MTs via its two structured doublecortin (DC) domains, but the dynamics of this

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