NIH 3T3 Cell Line murine
93061524, mouse embryo, Fibroblast
Synonym(s):
3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
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About This Item
UNSPSC Code:
41106514
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product name
NIH 3T3 Cell Line murine, 93061524
biological source
mouse embryo
description
Embryonic fibroblast
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Swiss NIH embryo
Cell Line Description
Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Application
DNA transfection studies
NIH 3T3 Cell Line has been used to:
- study calcium-mediated actin reset (CaAR) in response to physiological changes
- study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
- determine the viral titer of the retroviral construct -ST40hTNFα
Culture Medium
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
Other Notes
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Certificates of Analysis (COA)
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