Sp2/0-Ag14-TurboDoma
3092501, mouse unknown/unspecified, Lymphoblast
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About This Item
UNSPSC Code:
41106514
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product name
Sp2/0-Ag14-TurboDoma, 03092501
biological source
mouse unknown/unspecified
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma, non-producing, serum-free
Cell Line Description
Sp2/0-Ag14 cell line (Sigma Catalogue number. 85072401) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma (Cell Culture Technologies). Suitable for use as a fusion parent for serum-free generation of hybridomas as well as a host for the production of recombinant proteins.
Application
Fusion partner for generation of serum-free hybridomas, host for the expression of recombinant genes.
Culture Medium
TurboDoma™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.
Subculture Routine
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C..
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
TurboDoma is a trademark of Cell Culture Technologies
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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