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CA201

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 96 assays

Quality Level

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CAMP(820)

General description

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Application

cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.

Other Notes

Please refer to the attached Protocolfor details.

Kit Components Only

Product No.
Description

  • Acetic Anhydride 1 x 1

  • Assay Buffer 2 1 x 30

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP Assay Layout Sheet 1 x 1

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

See All (13)

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

Storage Class Code

3 - Flammable liquids


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Cyclic Nucleotide Signaling and the Cardiovascular System, 148-148 (2018)
Soumya Mukherjee et al.
Frontiers in microbiology, 7, 596-596 (2016-06-01)
This study catalogs production of industrially important enzymes and changes in transcript expression caused by 2-deoxy D-glucose (2-DG) treatment in Arthrinium malaysianum cultures. Carbon Catabolite Repression (CCR) induced by 2-DG in this species is cAMP independent unlike many other organisms.
Monika Schmoll et al.
Eukaryotic cell, 8(3), 410-420 (2009-01-13)
Although the enzymes enabling Hypocrea jecorina (anamorph Trichoderma reesei) to degrade the insoluble substrate cellulose have been investigated in some detail, little is still known about the mechanism by which cellulose signals its presence to the fungus. In order to
Role of beta3-adrenoceptors for intrahepatic resistance and portal hypertension in liver cirrhosis
Trebicka J, et al.
Hepatology, 50(6), 1924-1935 (2009)
Lingyue Yuan et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 40(10), 1465-1471 (2020-10-30)
To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene

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