C-39439
Adipocyte Nutrition Medium
SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml
Synonym(s):
Recommended with C-27431
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Recommended Products
form
liquid
packaging
pkg of 1 kit
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Adipocyte Nutrition Medium SupplementPack (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-27431
To form a complete medium, it should be combined with the basal medium: C-27431
Application
Adipocyte Nutrition Medium can be used to cultivate mature adipocytes. The medium is optimized for primary human cells but can also be used for murine adipocytes.Recommended for:Human Adipocytes
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.03 ml / mld-Biotin: 8 µg / mlInsulin (recombinant human): 0.5 µg / mlDexamethasone: 400 ng / ml
Storage and Stability
Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
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recommended
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Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
Flash Point(F)
55.4 °F
Flash Point(C)
13 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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