COLLDISP-RO
Roche
Collagenase/Dispase®
lyophilized, non-sterile, optimum pH 7.0-8.0
Synonym(s):
collagenase
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About This Item
UNSPSC Code:
12352204
Recommended Products
biological source
enzyme from Bacillus polymyxa ( Dispase )
enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
Quality Level
sterility
non-sterile
form
lyophilized
collagenase activity
>0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)
specific activity
>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
packaging
pkg of 100 mg (10269638001)
pkg of 500 mg (11097113001)
manufacturer/tradename
Roche
concentration
1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
technique(s)
tissue processing: suitable
color
light yellow to brown
optimum pH
7.0-8.0
solubility
water: soluble
UniProt accession no.
application(s)
life science and biopharma
sample preparation
foreign activity
proteolytic activities <0.1 U/mg (hemoglobin as the substrate)
storage temp.
2-8°C
Related Categories
General description
Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.
Specificity
Collagenase degrades native collagen. Dispase® is a nonspecific protease.
Application
Collagenase/Dispase® is used for the preparation of cells from a wide variety of different tissues and organs.
Components
EC 3.4.24.4 & 3.4.24.3
Unit Definition
One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.
Preparation Note
Activator: Ca2+
Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)
Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS.
Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)
Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS.
Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Reconstitution
Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca2+/Mg2+-free).
Storage and Stability
Store dry
Other Notes
Collagenase from Vibrio alginolyticus, Dispase® from Bacillus polymyxa
For life science research only. Not for use in diagnostic procedures.
Legal Information
Dispase is a registered trademark of Godo Shusei Co., Ltd.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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