03353583910

DIG Oligonucleotide Tailing Kit, 2^nd generation

sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)

• UNSPSC Code: 41105500
• NACRES: NA.55

Properties

• usage: sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
• Quality Level: 100
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: Aligned,

Description

General description

DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in:

• northern blot assay
• in situ hybridization (ISH)
• fluorescence in situ hybridization (FISH)

In addition to common hybridization techniques, DIG-labeled oligonucleotides are especially useful for screening expression libraries for sequence-specific DNA binding proteins, for example, transcription factors.

Features and Benefits

Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).

• Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
• Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells

Packaging

1 kit containing 11 components

Principle

DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).

Preparation Note

Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.

Storage and Stability

Store at -15–-25 °C. (unopened kit)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Reaction Buffer 5x concentrated
• CoCl<SUB>2</SUB> Solution 25 mM
• DIG-dUTP Solution 1 mM
• dATP Solution 10 mM
• Recombinant Terminal Transferase 400 U/μl
• Control Oligonucleotide, unlabeled 20 pmol/μl
• Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl
Control DNA 0.25 mg/ml
Glycogen Solution 20 mg/ml
DNA Dilution Buffer, 50 μg/ml fish sperm DNA
Poly(A) Solution 10 mg/ml

See All (11)

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332 - H350i - H360F - H411
• Precautionary Statements: P201 - P261 - P273 - P280 - P308 + P313 - P391
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash