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MABT822

Sigma-Aldrich

Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb11

clone mAB11, from rat

Synonym(s):

CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mAB11, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ITGA5(3678)
mouse ... Itga5(16402)

Related Categories

General description

Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated in B cells. It is composed of a light and heavy chain. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 4-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serves as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).

Specificity

Clone mAB11 is a rat monoclonal antibody that specifically detects Integrin alpha 5 in human and murine cells.

Immunogen

Fibronectin receptor purified from human placenta.

Application

Anti-Integrin alpha5 (CD49e), clone mAb11, Cat. No. MABT822 is a rat monoclonal antibody that detects Integrin alpha 5 and has been tested for use in Immunocytochemistry and Immunoprecipitation.
Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47; Clark, K., et. al. (2005). J Cell Sci. 118(Pt 2):291-300; Joo, E.E., et. al. (2014). Nat Commun. 5:3510).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47).
Research Category
Cell Structure

Quality

Evaluated by Immunocytochemistry in human Foreskin Fibroblast (HFF) cells.

Immunocytochemistry Analysis): A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.

Target description

114.54 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nishanth Belugali Nataraj et al.
Cell reports, 38(8), 110418-110418 (2022-02-24)
By establishing multi-omics pipelines, we uncover overexpression and gene copy-number alterations of nucleoporin-93 (NUP93), a nuclear pore component, in aggressive human mammary tumors. NUP93 overexpression enhances transendothelial migration and matrix invasion in vitro, along with tumor growth and metastasis in animal
Christoph J Burckhardt et al.
Developmental cell, 56(8), 1164-1181 (2021-03-25)
Cells probe their surrounding matrix for attachment sites via integrins that are internalized by endocytosis. We find that SH3BP4 regulates integrin surface expression in a signaling-dependent manner via clathrin-coated pits (CCPs). Dephosphorylated SH3BP4 at S246 is efficiently recruited to CCPs

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