MABS1152
Anti-ROR2 Antibody, clone Nt 2535-2835
clone Nt2535-2835, from mouse
Synonym(s):
Tyrosine-protein kinase transmembrane receptor ROR2, mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2
About This Item
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Nt2535-2835, monoclonal
species reactivity
mouse, human
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
wet ice
General description
Specificity
Immunogen
Application
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in various regions of wild-type and Ror2+/-, but not Ror2-/-, mouse E13.5 embryos by both fluorescent and non-fluorescent immunohistochemistry using paraformaldehyde-fixed, paraffin-embedded whole mount sections (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in the dermis of wild-type, but not Ror2-knockout mouse skin from E16.5 mouse embryos using paraformaldehyde-fixed, paraffin-embedded whole mount sections (van Amerongen, R., et al. (2012). Dev. Biol. 369(1):101-114).
Western Blotting Analysis: A representative lot detected full-length murine Ror2, as well as Ror2 deletion constructs lacking the first Ser/Thr-rich domain (ST1; a.a 753-782) or the Pro-rich domain (a.a. 784-857), but not Ror2 deletion mutants lacking the ST2 (a.a. 859-882) domain (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Enzyme-linked Immunoabsorbent Assay (ELISA): The immunoreactivity of a representative lot toward the immunogen MBP fusion protein was confirmed by ELISA (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Signaling
Kinases & Phosphatases
Quality
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ROR2 in 10 µg of MEF-1 cell lysate.
Target description
Physical form
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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