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Key Documents

MAB380

Sigma-Aldrich

Anti-β-Tubulin III Antibody

CHEMICON®, mouse monoclonal, 5H1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Tubulin β Antibody, clone 5H1, ascites fluid, clone 5H1, Chemicon®

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5H1, monoclonal

species reactivity

rat, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TTLL8(164714)

Specificity

Beta tubulin

Immunogen

Rat tubulin.

Application

Anti-Tubulin Antibody, β, clone 5H1 is an antibody against Tubulin for use in IH.
Immunohistochemistry at 1:300-1:1,000

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380

This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Physical form

Ascites fluid containing sodium azide. Liquid

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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