AP181P
Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed
0.8 mg/mL, Chemicon®
Synonym(s):
HRP-Conjugated Goat Anti-Mouse IgG
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
concentration
0.8 mg/mL
technique(s)
ELISA: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Application
EIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
This Goat anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Mouse IgG.
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Shuyu Hao et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 19(1), 34-39 (2019-05-28)
Individuals with cystic fibrosis (CF) experience elevated inflammation in multiple organs, but whether this reflects an inherent feature of CF cells or is a consequence of a pro-inflammatory environment is not clear. Using CRISPR/Cas9-mediated mutagenesis of CFTR, 17 subclonal cell
Efficacy of epidermal growth factor receptor-targeted molecular therapy in anaplastic thyroid cancer cell lines.
Nobuhara, Y; Onoda, N; Yamashita, Y; Yamasaki, M; Ogisawa, K; Takashima, T; Ishikawa, T; Hirakawa, K
British Journal of Cancer null
Bhupal P Bhetwal et al.
The Journal of physiology, 591(12), 2971-2986 (2013-04-25)
Ca(2+) sensitization of contraction has typically been investigated by bathing muscles in solutions containing agonists. However, it is unknown whether bath-applied agonists and enteric neurotransmission activate similar Ca(2+) sensitization mechanisms. We investigated protein kinase C (PKC)-potentiated phosphatase inhibitor protein of
Chiho Kim et al.
eLife, 9 (2020-08-28)
It is being increasingly appreciated that the immunomodulatory functions of PARP1 inhibitors (PARPi) underlie their clinical activities in various BRCA-mutated tumors. PARPi possess both PARP1 inhibition and PARP1 trapping activities. The relative contribution of these two mechanisms toward PARPi-induced innate
Irina Naletova et al.
Oncotarget, 9(91), 36289-36316 (2018-12-18)
Copper homeostasis is generally investigated focusing on a single component of the metallostasis network. Here we address several of the factors controlling the metallostasis for neuroblastoma cells (SH-SY5Y) upon treatment with 2,9-dimethyl-1,10-phenanthroline-5,6-dione (phendione) and 2,9-dimethyl-1,10-phenanthroline (cuproindione). These compounds bind and
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service