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AB1252

Sigma-Aldrich

Anti-Cytochrome P450 Enzyme CYP2E1 Antibody

serum, Chemicon®

Synonym(s):

cytochrome P450 2E1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP2E1(1571)
mouse ... Cyp2E1(13106)
rat ... Cyp2E1(25086)

Related Categories

General description

Cytochrome P450 (CYP) is a huge and diverse superfamily of hemoproteins. It metabolize the fatty acid arachidonic acid (AA) to regulators of vascular tone.
Most CYPs can metabolize multiple substrates, and can catalyze multiple reactions. This accounts for their central importance in metabolizing the extremely large number of endogenous and exogenous molecules. In the liver, these substrates include drugs and toxic compounds as well as metabolic products such as bilirubin (a breakdown product of hemoglobin). Cytochrome P450 enzymes are present in many other tissues of the body including the mucosa of the gastrointestinal tract, and play important roles in hormone synthesis and breakdown (including estrogen and testosterone synthesis and metabolism), cholesterol synthesis, and vitamin D metabolism. More than 7700 distinct CYP sequences are known as of September 2007.

Specificity

Reacts with human and rat cytochrome P450 enzyme CYP2E1 in hepatic microsomal fractions. No cross-reactivity with other cytochrome P450 enzymes in these species.

Application

Anti-Cytochrome P450 Enzyme CYP2E1 Antibody is an antibody against Cytochrome P450 Enzyme CYP2E1 for use in IH & WB.
Immunohistochemistry:
A previous lot was used on formaldehyde treated and frozen sections.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by Western Blot on Human brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Cytochrome P450 CYP2E1 on 10 μg of Human brain lysates.

Target description

57 kDa

Physical form

Rabbit polyclonal antiserum in buffer containing no preservative.

Analysis Note

Control
Liver tissue.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kyle J Thompson et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 52(6), 629-637 (2017-10-17)
This study sought to compare mice bred to preferentially consume high amounts of alcohol (crossed-high alcohol preferring, cHAP) to c57BL/6 (C57) mice using a chronic-binge ethanol ingestion model to induce alcoholic liver disease (ALD). Male C57 and cHAP mice were
Rebecca L McCullough et al.
Molecular immunology, 75, 122-132 (2016-06-10)
Complement is implicated in the development of alcoholic liver disease. C3 and C5 contribute to ethanol-induced liver injury; however, the role of C5a receptor (C5aR) on myeloid and non-myeloid cells to progression of injury is not known. C57BL/6 (WT), global
Pengcheng Wang et al.
Biochemical pharmacology, 145, 218-225 (2017-09-11)
Acetylation is the major metabolic pathway of isoniazid (INH) mediated by N-acetyltransferases (NATs). Previous reports suggest that slow acetylators have higher risks of INH hepatotoxicity than rapid acetylators, but the detailed mechanisms remain elusive. The current study used Nat1/2(-/-) mice
Xiaoxia Jin et al.
Frontiers in pharmacology, 9, 1317-1317 (2018-12-14)
This study was designed to explore the role of cytochrome P4502E1 (CYP2E1) expression in the course of brain edema induced by subacute poisoning with 1,2-dichloroethane (1,2-DCE). Mice were randomly divided into five groups: the control group, the 1,2-DCE poisoned group
Murali Ganesan et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 101-112 (2018-04-26)
Alcohol-induced progression of hepatitis C virus (HCV) infection is related to dysfunction of innate immunity in hepatocytes. Endogenously produced interferon (IFN)α induces activation of interferon-stimulated genes (ISGs) via triggering of the Janus kinase-signal transducer and activator of transcription 1 (STAT1)

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