Skip to Content
Merck
All Photos(1)

Documents

400050

Millipore

Hygromycin B, Streptomyces sp., Cell Culture-Tested

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.

Synonym(s):

Hygromycin B, Streptomyces sp., Cell Culture-Tested

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
Molecular Weight:
527.52
MDL number:
UNSPSC Code:
51102829

Quality Level

Assay

≥90% (HPLC and TLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

light brown

solubility

distilled water: ≥100 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1

InChI key

GRRNUXAQVGOGFE-KPBUCVLVSA-N

General description

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).



Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).



The analytical data listed below will vary from lot to lot.



Appearance: Off-white to light brown solid

Bioassay: 1000 U/mg

Solubility: ≥100 mg/ml in H2O

Purity (HPLC): ≥90%

Cytotoxicity: Tested in HeLa and/or CHO cells
Potency: ≥1000 µ/mg solid.
Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Other Notes

Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Bilang, R., et al. 1991. Gene100, 247.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jens Hamar et al.
Scientific reports, 11(1), 7854-7854 (2021-04-14)
CRISPR/Cas9 gene editing is effective in manipulating genetic loci in mammalian cell cultures and whole fish but efficient platforms applicable to fish cell lines are currently limited. Our initial attempts to employ this technology in fish cell lines using heterologous
Mei Xu et al.
STAR protocols, 2(4), 100881-100881 (2021-11-23)
Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain
Anna M van Ziel et al.
Journal of neurochemistry, 152(2), 208-220 (2019-08-24)
The unfolded protein response (UPR) is one of the major cell-autonomous proteostatic stress responses. The UPR has been implicated in the pathogenesis of neurodegenerative diseases and is therefore actively investigated as therapeutic target. In this respect, cell non-autonomous effects of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service