07-888

Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

Upstate^®, from rabbit

• Synonym(s): 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001092102
• UniProt accession no.: Q96B36
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr246)
• Gene Information: human ... AKT1S1(84335) ; mouse ... Akt1S1(67605)

Description

General description

PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

Specificity

Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.

Immunogen

Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).

Application

Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.

Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.

Quality

Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.

Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.

Target description

Approx. 40 kDa

Physical form

Format: Purified

Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.

Analysis Note

Control
PDGF stimulated NIH3T3 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2